abstract：:Following cloning of Leishmania (L.) amazonensis kinetoplast DNA two recombinant clones were identified: one specific for L. (L.) amazonensis and the other specific for L. (L.) amazonensis and closely related isolates. DNA sequences from these clones were compared with those of other kinetoplastids and oligonucleotide...

journal_title：Parasitology

authors： Eresh S,McCallum SM,Barker DC

更新日期：1994-11-01 00:00:00

abstract：:A novel repetitive DNA sequence in the sheep parasitic nematode Trichostrongylus colubriformis was cloned and sequenced. A 1.1 kb repetitive sequence (Tc15) which hybridized with DNA from T. colubriformis but not with DNA from two other parasitic nematodes, Haemonchus contortus and Ostertagia circumcincta, or sheep wa...

journal_title：Parasitology

authors： Callaghan MJ,Beh KJ

更新日期：1994-09-01 00:00:00

abstract：:The presence of superoxide dismutase (SOD) activity in the bovine lungworm Dictyocaulus viviparus was examined using the xanthine-xanthine oxidase assay system and by non-denaturing PAGE followed by specific enzyme staining. High levels of activity were detected in excretory-secretory (ES) products of adult worms and ...

journal_title：Parasitology

authors： Britton C,Knox DP,Kennedy MW

更新日期：1994-08-01 00:00:00

abstract：:Receptors interacting with terminal sugars as ligands are involved in the binding of Leishmania donovani promastigotes to the macrophage surface and their subsequent internalization. Mannose and glucose are specifically involved in the binding process. Decreased binding occurs to macrophages already infected with L. d...

journal_title：Parasitology

authors： Dutta M,Bandyopadhyay R,Basu MK

更新日期：1994-08-01 00:00:00

abstract：:We investigated changes in the behaviour of Stagnicola elodes associated with the emergence of Plagiorchis elegans cercariae. Within 15 min of the reduction in light intensity, which triggered the onset of cercarial emergence, infected snails moved to the top of the water column and remained there for 2-3 h. Seventy-n...

journal_title：Parasitology

authors： Lowenberger CA,Rau ME

更新日期：1994-07-01 00:00:00

abstract：:The transmission-blocking monoclonal antibody 13.1, which recognizes the ookinete surface antigen Pbs21 of Plasmodium berghei, and an IgG2a isotype control antibody 26.37 were purified by caprylic acid and ammonium sulphate precipitation. Fab fragments were prepared by papain digestion. IgG but not Fab from antibody 1...

journal_title：Parasitology

authors： Ranawaka GR,Alejo-Blanco AR,Sinden RE

更新日期：1994-07-01 00:00:00

abstract：:Theileria-free waterbuck (Kobus defassa) born in captivity were successfully infected with Theileria parva sporozoites derived from ticks infected by feeding on African buffalo (Syncerus caffer). All waterbuck underwent mild infections with the development of sporadic schizont and piroplasm parasitosis when inoculated...

journal_title：Parasitology

authors： Stagg DA,Bishop RP,Morzaria SP,Shaw MK,Wesonga D,Orinda GO,Grootenhuis JG,Molyneux DH,Young AS

更新日期：1994-06-01 00:00:00

abstract：:The primary immune response to infection with Heligmosomoides polygyrus was studied in mice differing in response phenotype (fast-SWR, intermediate-NIH, slow-CBA). Marked IgG1 and IgE but not IgG2a antibody responses were detected in infected mice and the former were more intense in fast compared with slow responder s...

journal_title：Parasitology

authors： Wahid FN,Behnke JM,Grencis RK,Else KJ,Ben-Smith AW

更新日期：1994-05-01 00:00:00

abstract：:Intraerythrocytic malaria parasites ingest the cytosol of their host cell and digest it inside their acid food vacuoles. Acidified (pH 4-5.5, 37 degrees C) human red blood cell lysates were used to simulate this process, measuring the denaturation of haemoglobin (Hb) and the release of iron, in the absence or presence...

journal_title：Parasitology

authors： Gabay T,Krugliak M,Shalmiev G,Ginsburg H

更新日期：1994-05-01 00:00:00

abstract：:We studied the component community of lung-worms of the harbour porpoise, attempting to establish the relative importance of ecological and evolutionary factors on its development. The lungs of 64 porpoises by-caught in Norwegian waters were examined for helminths. Three pseudaliid species were detected. The porpoises...

journal_title：Parasitology

authors： Balbuena JA,Aspholm PE,Andersen KI,Bjørge A

更新日期：1994-04-01 00:00:00

abstract：:A striking feature of toxoplasmic seroconversion is the prominent and early IgM response to a low molecular weight antigen of 4-5 kDa. Two different monoclonal antibodies directed against the 4-5 kDa antigen have been generated and used to characterize this molecule. Using these monoclonal antibodies, we could demonst...

journal_title：Parasitology

authors： Tomavo S,Couvreur G,Leriche MA,Sadak A,Achbarou A,Fortier B,Dubremetz JF

更新日期：1994-02-01 00:00:00

abstract：:Acetylcholinesterase (AChE) is present in all stages of the life-cycle of schistosomes and is located in muscle and on the surface of the parasite. Metrifonate is a drug that inhibits AChE. We compared the AChEs from three schistosome species (Schistosoma mansoni, Schistosoma haematobium and Schistosoma bovis) that ha...

journal_title：Parasitology

authors： Camacho M,Tarrab-Hazdai R,Espinoza B,Arnon R,Agnew A

更新日期：1994-02-01 00:00:00

abstract：:The transmission success of Onchocerca volvulus is thought to be influenced by a variety of regulatory or density-dependent processes that act at various points in the two-host life-cycle. This paper examines one component of the life-cycle, namely, the ingestion of microfilariae by the simuliid vector, to assess the ...

journal_title：Parasitology

authors： Basáñez MG,Boussinesq M,Prod'hon J,Frontado H,Villamizar NJ,Medley GF,Anderson RM

更新日期：1994-01-01 00:00:00

abstract：:Host location by parasites can be achieved by either active or passive mechanisms. In spite of their significance, the efficacy of these methods has been little researched. High fecundity in parasites is discussed in terms of the role it plays in dispersal and transmission. Some concepts developed by mainstream behavi...

journal_title：Parasitology

authors： Rea JG,Irwin SW

更新日期：1994-01-01 00:00:00

abstract：:The multicopy mini-exon-derived RNA (med RNA) locus of Leishmania donovani was enzymatically amplified by the polymerase chain reaction (PCR). The major 180 bp PCR product contained conserved med RNA gene sequences flanking the variable intergenic spacer from the med RNA gene tandem repeat. The oligonucleotide primers...

journal_title：Parasitology

authors： Hassan MQ,Ghosh A,Ghosh SS,Gupta M,Basu D,Mallik KK,Adhya S

更新日期：1993-12-01 00:00:00

abstract：:Isolates of Trypanosoma cruzi from human patients, domestic and sylvatic animals and vector insects were obtained in different areas of Argentina. Electrophoretic patterns of enzymes from extracts of 95 isolates were analysed. On the basis of zymograms providing information on 10 loci, 12 zymodemes are described accor...

journal_title：Parasitology

authors： de Luca D'oro GM,Gardenal CN,Perret B,Crisci JV,Montamat EE

更新日期：1993-11-01 00:00:00

abstract：:The lung schistosomulum of Schistosoma mansoni is the target of protective immunity in mice singly vaccinated with irradiated cercariae. Since the effector responses are T cell-mediated, their initiation requires the release of antigens from the intact parasite. We have used the technique of biosynthetic labelling wit...

journal_title：Parasitology

authors： Harrop R,Wilson RA

更新日期：1993-09-01 00:00:00

abstract：:The complete small subunit ribosomal RNA (srRNA) gene of Theileria parva was cloned and sequenced. Two primers were designed which permitted the specific amplification of part of the Theileria srRNA gene from Theileria-infected cell line samples which were predominantly (> 95%) bovine DNA. The sequence of the central ...

journal_title：Parasitology

authors： Allsopp BA,Baylis HA,Allsopp MT,Cavalier-Smith T,Bishop RP,Carrington DM,Sohanpal B,Spooner P

更新日期：1993-08-01 00:00:00

abstract：:A dipstick dot ELISA for detection of Taenia-specific coproantigens was developed. The test was based on a sandwich ELISA using antibodies raised against adult Taenia solium. Antibodies were absorbed to nitrocellulose paper previously adhered to acetate plastic to form dipsticks. Once blocked with 5% skimmed milk and ...

journal_title：Parasitology

authors： Allan JC,Mencos F,Garcia-Noval J,Sarti E,Flisser A,Wang Y,Liu D,Craig PS

更新日期：1993-07-01 00:00:00

abstract：:A recombinant baculovirus-expressed hybrid protein containing epitopes for the C-terminal fragment of the Plasmodium falciparum precursor to the major merozoite surface antigens (PMMSA) and the tetrapeptide repeats of the circumsporozoite protein (CSP) was assessed for its immunogenicity. Murine MHC-II restriction of ...

journal_title：Parasitology

authors： Lockyer MJ,Cooper H,Tite J,Rowan W,Crowe JS

更新日期：1993-06-01 00:00:00

abstract：:Giardia lamblia localize and multiply in the small intestine and may cause acute or chronic diarrhoea with malabsorption of fat, protein and other nutrients. Abnormal pancreatic function has been documented in giardiasis and trophozoites directly inhibit pancreatic lipase activity in vitro. The aim of this study was t...

journal_title：Parasitology

authors： Seow F,Katelaris P,Ngu M

更新日期：1993-04-01 00:00:00

abstract：:The snail Bulinus nasutus 1214 possesses a potent haemagglutinin (end-point titre with human erythrocytes, 2(-18)) in its cell-free haemolymph which also binds to the miracidia (but not other larvae) of the incompatible parasite Schistosoma margrebowiei. We have purified a protein possessing this haemagglutinating pro...

journal_title：Parasitology

authors： Harris RA,Preston TM,Southgate VR

更新日期：1993-02-01 00:00:00

abstract：:Adults of the porocephalid pentastomid Porocephalus crotali infect the lung of rattlesnake definitive hosts and larvae develop in rat intermediate hosts. In the latter, nymphs encyst within a variety of tissue sites (commonly abdominal fat bodies and lungs) and each becomes the focus of an eosinophilic granuloma. From...

journal_title：Parasitology

authors： McHardy P,Riley J,Huntley JF

更新日期：1993-01-01 00:00:00

abstract：:Susceptibility to oxidative stress is a well-established feature of the malarial parasite. Pharmacologists have taken advantage of this property to design highly effective pro-oxidant antimalarial drugs. Less well appreciated is the fact that nutritional manipulation of host oxidative stress status by dietary means ca...

journal_title：Parasitology

authors： Levander OA,Ager AL Jr

更新日期：1993-01-01 00:00:00

abstract：:Stratospheric ozone depletion threatens to increase exposure to ultraviolet (UV) radiation which is known to be a factor in a number of diseases. There is little doubt that cumulative exposure to UV radiation is important in the aetiology of non-melanoma skin cancers. Evidence is also strong for a link with cutaneous ...

journal_title：Parasitology

authors： Bentham G

更新日期：1993-01-01 00:00:00

abstract：:Potential mechanisms of immunoregulation have been investigated for the capacity to generate heterogeneity in the outcome of infection with helminth parasites. We have developed a mathematical model of the interaction between T cell and parasite populations, based on the assumption that activation of a Th1 CD4+ T cell...

journal_title：Parasitology

authors： Schweitzer AN,Anderson RM

更新日期：1992-12-01 00:00:00

abstract：:Cells of Leishmania donovani in co-culture with Trypanosoma brucei, were severely affected in their growth, resulting in swelling and subsequent lysis. Similar effects were also observed when Crithidia luciliae or Phytomonas sp. were co-cultured with T. brucei. Direct contact between the cells under investigation and ...

journal_title：Parasitology

authors： Coppens I,Ter Kuile BH,Opperdoes FR

更新日期：1992-12-01 00:00:00

abstract：:Fractionation of Schistosoma mansoni cercariae gland secretion on a Sephadex G-150 column followed by a Superose-12 column in an FPLC system, isolated a 47 kDa protease which migrated as a single band on SDS-PAGE gels. A monoclonal antibody (MAb) was produced which recognizes only the 47 kDa protease, and an immuno-af...

journal_title：Parasitology

authors： Chavez-Olortegui C,Resende M,Tavares CA

更新日期：1992-10-01 00:00:00

abstract：:Clones of Trypanosoma congolense which express resistance to the widely used trypanocide isometamidium chloride accumulate less of the drug than clones which are sensitive to drug treatment. A mathematical model has been developed which was able to predict theoretical lines representing the uptake kinetics in trypanos...

journal_title：Parasitology

authors： Sutherland IA,Mounsey A,Eisler M,Holmes PH

更新日期：1992-08-01 00:00:00

abstract：:Laboratory experiments indicated that the active life-span of the cercariae of Cryptocotyle lingua was temperature-dependent. An increase in water temperature and population age both correlated with a tendency for the organisms to become decaudate. The larvae were strongly photoresponsive to lateral light but with tim...

journal_title：Parasitology

authors： Rea JG,Irwin SW

更新日期：1992-08-01 00:00:00

abstract：:A 196 bp fragment of cloned kinetoplast (k) DNA of Leishmania infantum has been sequenced and shown to be diagnostic for this species. The DNA from 10(4)-10(5) promastigotes was routinely and specifically detected in crude dot blots of whole cells lysed in situ, and there was no cross-hybridization with 10(6) cells of...

journal_title：Parasitology

authors： Gramiccia M,Smith DF,Angelici MC,Ready PD,Gradoni L

更新日期：1992-08-01 00:00:00

abstract：:The importance of host heterogeneity in generating aggregation was investigated using Heligmosomoides polygyrus (Nematoda) in laboratory mice. Parameters of infection were compared between inbred and outbred mice, between primary and challenge infection protocols, and between gavage and natural exposure protocols, to ...

journal_title：Parasitology

authors： Tanguay GV,Scott ME

更新日期：1992-06-01 00:00:00

abstract：:Cats were repeatedly inoculated with infective larvae of Brugia pahangi. On parasitological grounds they could be divided into 5 groups. Group I--most cats (some 70%) became microfilaraemic (mf+) and retained high levels of microfilariae (mf) in their blood for over 2 years. In some Group I cats mf counts stabilized a...

journal_title：Parasitology

authors： Denham DA,Medeiros F,Baldwin C,Kumar H,Midwinter IC,Birch DW,Smail A

更新日期：1992-06-01 00:00:00

abstract：:Responses to Taenia taeniaeformis infection were studied in mice with severe combined immunodeficiency (scid), which lack functional T and B lymphocytes. In the early phase of infection, accumulation of polymorphonuclear leucocytes (PML) occurred around the larvae in the liver of scid mice and their immunocompetent co...

journal_title：Parasitology

authors： Ishiwata K,Oku Y,Ito M,Kamiya M

更新日期：1992-04-01 00:00:00

abstract：:Recombinant ribosomal DNA sequences were amplified by PCR and used as probes to perform a fingerprint analysis of total DNA from different Entamoeba histolytica isolates. RFLPs obtained with one of the probes, R-1, support previous proposals that pathogenic and non-pathogenic E. histolytica are closely related, yet ge...

journal_title：Parasitology

authors： Cruz-Reyes JA,Spice WM,Rehman T,Gisborne E,Ackers JP

更新日期：1992-04-01 00:00:00

abstract：:The localization of adult Schistosoma mansoni originating from Guadeloupe (West Indies) was analysed in the natural host Rattus rattus in experimental and natural infections. A transfer of schistosomes from the porto-mesenteric system to the lungs occurs between the 4th and the 20th weeks post-infection, with a peak b...

journal_title：Parasitology

authors： Imbert-Establet D,Combes C

更新日期：1992-02-01 00:00:00

abstract：:Ultrastructural investigations (SEM, TEM) combined with lectin-binding analysis, have revealed concurrent modifications in tegumentary structure and surface glycoconjugates during the establishment and differentiation of Echinococcus multilocularis metacestodes in jirds. The laminated layer, which is amorphous and ric...

journal_title：Parasitology

authors： Leducq R,Gabrion C

更新日期：1992-02-01 00:00:00

abstract：:In situations where malaria eradication is not an option in the foreseeable future the emphasis must be on the control of morbidity and mortality due to malaria. Under such circumstances drawing a distinction between malarial parasitization and malarial disease may be important for workers in both field and laboratory...

journal_title：Parasitology

authors： Marsh K

更新日期：1992-01-01 00:00:00

abstract：:This paper reviews naturally occurring and synthetic compounds that either enhance immune defences or lower both natural and acquired immunity. Immunomodulatory agents used both for laboratory study and clinically for the management of immunologically based diseases are considered. ...

journal_title：Parasitology

authors： Nüssler AK,Thomson AW

更新日期：1992-01-01 00:00:00

abstract：:BALB/c mice were characterized as more resistant to infection with Heligmosomoides polygyrus (Nematoda) than C57BL/6 mice based on lower establishment and survival during a primary infection and stronger protection induced by an immunizing regime. It was hypothesized, therefore, that C57BL/6 mice would be more heavily...

journal_title：Parasitology

authors： Scott ME

更新日期：1991-12-01 00:00:00